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ObjectiveA method was developed for the rapid determination of 18 common disinfection by-products including halogenated oxides and haloacetic acid （HAAs） in drinking water by high performance liquid chromatography tandem mass spectrometry （HPLC-MS/MS）. MethodThe water sample was filtered by 0.22 μm hydrophilic membrane then the analytes were separated on a PFP （2.1 mm× 100 mm， 2.7 μm） pentafluorophenyl column with 0.1% acetic acid and acetonitrile as mobile phase gradient elution. Ionization in anionic electrospray mode was detected by multi-reaction monitoring （MRM） mode. The external standard method was used for quantitation. ResultsThe correlation coefficients of 18 disinfection by-products were above 0.999 in the corresponding linear range. The average spiked recoveries of 1， 10 and20 times of LOQ of each analyte were 91.6%‒101.8%， and the relative standard deviation （RSD） was 1.2%‒6.4%. The LOD and LOQ were 0.020‒2 μg·L-1 and 0.050‒5 μg·L-1， respectively. ConclusionThis method is simple， sensitive and accurate， and could be used for the routine analysis of 18 common disinfection by-products in drinking water.
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Objective To observe the clinical curative effect of diosmin combined with nimesulide in treatment of drugs for the traumatic synovitis ofin knee joint.Methods Sixty patients the People's Liberation Army 425 Hospital diagnosis standard and the requirements in this study were averagely and randomly divided into combination group,nimesulide group and diosmin group,the patients in combination group were given diosmin and nimesulide treatment,the patients in nimesulide group were given nimesulide treatment,diosmin group were given diosmin treatment.The course of treatment was 14 d.Then score knee joint VAS,AKS rating,and swelling degree in each group before and after the treatment.Results After treatment,the patients with knee joint swelling,pain degree are improved,the difference before and after treatment has statistical significance (P ＜ 0.05),meanwhile differences in the combination group were larger than the other two groups (P ＜ 0.05).Conclusion The drugs of nimesulide and diosmin for the treatment of traumatic synovitis respectively have certain effect,but the combined treatment curative effect is remarkable than used alone.Combination medication can be more obviously relieving the pain of patients and prevent disease progression,reduce inflammation which achieved a satisfactory therapeutic effect.
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Objective:To establish an HPLC method to determine vanillin in 18 dairy products. Methods:The samples were sep-arated on a C18 column (250 mm × 4. 6 mm, 5 μm) with the mobile phase of methanol and water (35∶65) at the flow rate of 1. 0 ml ·min-1 . The detection wavelength was 275 nm. Results:The LOD ( limit of detection) of vanillin was 0. 45 μg·ml-1 . Excellent linearity was obtained within the concentration range of 1.990-99.500 μg·ml-1(r=1.000 0). The average recovery of vanillin in different dairy products with low, medium and high levels varied between 96. 26% and 100. 81% with RSD of 0. 15%-2. 22%. Con-clusion:The method is simple and reproducible, which can be applied in the rapid analysis of vanillin in dairy products.